Detection of expanded CAG repeats in Bipolar Affective Disorder using the repeat expansion detection (RED) method

Neurobiology of Disease

Christer Engström¹  Martin Schalling¹  Tom Hudson²  Per-Olof Nylander²  Daniel Sourey³  Julien Mendlewicz³  An De bruyn⁴  Jayanti Chotai⁴  Christine Van Broeckhoven⁵  Cecilia Zander⁵  Kerstin Lindblad⁶  Gösta Holmgren⁶  Rolf Adolfsson⁶ 

[1]Department of Clinical Genetics, University of Umeå, Sweden

[2]Department of Psychiatry, University of Umeå, Sweden

[3]Division of Psychiatry, Erasme Hospital, University of Brussels (ULB), Belgium

[4]Neurogenetics Laboratory, Born Bunge Foundation, Department of Biochemistry, University of Antwerp (UIA), B2610-Antwerp, Belgium

[5]Whitehead Institute, Cambridge, MA, U.S.A.

[6]Neurogenetics Unit, Department of Molecular Medicine, Karolinska Hospital, Stockholm, Sweden

关键词: affective disorders;    anticipation;    Bipolar disorder;    RED Method;    trinucleotide repeats;   

DOI  :  

来源: DOAJ

【 摘 要 】

Genetic factors are of major aetiological importance in Bipolar Affective Disorder (BPAD type I and II). The exact mode of inheritance of BPAD is unknown, but the recent demonstration of anticipation suggests that dynamic mutations could be involved in the clinical expression of the disease.We have used the repeat expansion detection (RED) method to test whether the anticipation in BPAD could be explained by the presence of expanded trinucleotide repeat sequences. Using a (CTG)10oligonucleotide a significantly higher number of expanded CAG repeats were found in the genomic DNA of two independent samples of unrelated BPAD patients of Swedish and Belgian ancestry as compared with normal controls. The difference in repeat number was more consistent if data of the two samples of patients was pooled.In this study a CAG trinucleotide repeat expansion was associated for the first time with a major psychiatric disorder. It is possible that the CAG trinucleotide repeat expansion is involved in the clinical expression of BPAD and that it is the molecular basis explaining the phenomenon of anticipation observed in this disorder.

【 授权许可】

Unknown