Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior

STAR Protocols

Marco Padilla-Rodriguez¹  Jillian A. Sweetland²  Samantha M. Hill²  Amber Clements³  Ghassan Mouneimne³  Noel A. Warfel³  Sara S. Parker⁴ 

[1] Corresponding author;Departent of Cancer Biology GIDP, University of Arizona, Tucson, AZ 85724, USA;Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA;Department of Science, University of Arizona, Tucson, AZ 85724, USA;

关键词: Biophysics;    Cell Biology;    Cancer;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: We describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion.For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

【 授权许可】

Unknown