Proteomics in the World of Induced Pluripotent Stem Cells

Cells

RafaelSoares Lindoso¹  Federica Collino¹  Adalberto Vieyra¹  Gustavo Monnerat Cahli¹  Adriana Bastos Carvalho¹  TaisH. Kasai-Brunswick¹  AntonioCarlos Campos de Carvalho¹ 

[1] Carlos Chagas Filho Institute of Biophysics and National Center for Structural Biology and Bioimaging/CENABIO, Federal University of Rio de Janeiro, Rio de Janeiro 21941-102, Brazil;

关键词: induced pluripotent stem cells;    proteomics;    embryonic stem cells;    differentiation;    organoids;   

DOI  :  10.3390/cells8070703

来源: DOAJ

【 摘 要 】

Omics approaches have significantly impacted knowledge about molecular signaling pathways driving cell function. Induced pluripotent stem cells (iPSC) have revolutionized the field of biological sciences and proteomics and, in particular, has been instrumental in identifying key elements operating during the maintenance of the pluripotent state and the differentiation process to the diverse cell types that form organisms. This review covers the evolution of conceptual and methodological strategies in proteomics; briefly describes the generation of iPSC from a historical perspective, the state-of-the-art of iPSC-based proteomics; and compares data on the proteome and transcriptome of iPSC to that of embryonic stem cells (ESC). Finally, proteomics of healthy and diseased cells and organoids differentiated from iPSC are analyzed.

【 授权许可】

Unknown