eLife | |
Rhabdo-immunodeficiency virus, a murine model of acute HIV-1 infection | |
Emily J Mastrocola1  Theodora Hatziioannou1  Fabian Schmidt1  Jessie R Willen2  Dennis Voronin2  Paul D Bieniasz2  Trinity M Zang2  Rachel A Liberatore2  Elena Cassella2  | |
[1] Howard Hughes Medical Institute, The Rockefeller University, New York, United States;Laboratory of Retrovirology, The Rockefeller University, New York, United States; | |
关键词: HIV-1; antibody; envelope; CD4; CCR5; neutralization; | |
DOI : 10.7554/eLife.49875 | |
来源: DOAJ |
【 摘 要 】
Numerous challenges have impeded HIV-1 vaccine development. Among these is the lack of a convenient small animal model in which to study antibody elicitation and efficacy. We describe a chimeric Rhabdo-Immunodeficiency virus (RhIV) murine model that recapitulates key features of HIV-1 entry, tropism and antibody sensitivity. RhIVs are based on vesicular stomatitis viruses (VSV), but viral entry is mediated by HIV-1 Env proteins from diverse HIV-1 strains. RhIV infection of transgenic mice expressing human CD4 and CCR5, exclusively on mouse CD4+ cells, at levels mimicking those on human CD4+ T-cells, resulted in acute, resolving viremia and CD4+ T-cell depletion. RhIV infection elicited protective immunity, and antibodies to HIV-1 Env that were primarily non-neutralizing and had modest protective efficacy following passive transfer. The RhIV model enables the convenient in vivo study of HIV-1 Env-receptor interactions, antiviral activity of antibodies and humoral responses against HIV-1 Env, in a genetically manipulatable host.
【 授权许可】
Unknown