Using Gold-Nanorod-Filled Mesoporous Silica Nanobeads for Enhanced Radiotherapy of Oral Squamous Carcinoma

Nanomaterials

Tse-Ying Liu¹  Chia-Ying Li¹  Mei-Hsiu Chen²  San-Yuan Chen³  Fu-I Tung⁴  Ming-Hong Chen⁵ 

[1] Department of Biomedical Engineering, National Yang Ming Chiao Tung University, Taipei 112, Taiwan;Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei 220, Taiwan;Department of Materials Science and Engineering, National Yang Ming Chiao Tung University, Hsinchu 300, Taiwan;Department of Orthopaedic Surgery, Taipei City Hospital, Taipei 112, Taiwan;Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 110, Taiwan;

关键词: radiotherapy;    mesoporous silica;    gold nanoparticles;    radiosensitizer;    oral cancer;   

DOI  :  10.3390/nano11092235

来源: DOAJ

【 摘 要 】

Radiotherapy (RT), in combination with surgery, is an essential treatment strategy for oral cancer. Although irradiation provides effective control over tumor growth, the surrounding normal tissues are almost inevitably affected. With further understanding of the molecular mechanisms involved in radiation response and recent advances in nanotechnology, using gold nanoparticles as a radiosensitizer provides the preferential sensitization of tumor cells to radiation and minimizes normal tissue damage. Herein, we developed gold nano-sesame-beads (GNSbs), a gold-nanorod-seeded mesoporous silica nanoparticle, as a novel radioenhancer to achieve radiotherapy with a higher therapeutic index. GNSbs in combination with 2 Gy irradiation effectively enhanced the cytotoxic activity CAL-27 cells. The well-designed structure of GNSbs showed preferential cellular uptake by CAL-27 cells at 24 h after incubation. Gold nanorods with high density modified on mesoporous silica nanoparticles resulted in significant reactive oxygen species (ROS) formation after irradiation exposure compared with irradiation alone. Furthermore, GNSbs and irradiation induced more prominent DNA double-strand breaks and G2/M phase arrest in CAL-27 than those in L929. In animal studies, radiotherapy using GNSbs as a radiosensitizer showed significant suppression of tumor growth in an orthotopic model of oral cancer. These results demonstrate that using GNSbs as a radiosensitizer could possess clinical potential for the treatment of oral squamous carcinoma.

【 授权许可】

Unknown