Chromosome structural variation of two cultivated tetraploid cottons and their ancestral diploid species based on a new high-density genetic map

Scientific Reports

Xue-ying Liu¹  Ai-ai Zhu¹  You-ming Tian¹  Xiao-mei Fang¹  De-xin Liu¹  Ya-qiong Xu¹  Zhao-yun Tan¹  Zheng-sheng Zhang¹  Rui Tian¹  Jiang Yao¹  Da-jun Liu¹  Jian Zhang¹  Wen-wen Wang¹  Yan Li¹  Zhong-hua Teng¹  Fang Liu²  Hai-hong Shang² 

[1] Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University;State Key Laboratory of Cotton Biology/Cotton Research Institute, Chinese Academy of Agricultural Sciences;

DOI  :  10.1038/s41598-017-08006-w

来源: DOAJ

【 摘 要 】

Abstract A high-resolution genetic map is a useful tool for assaying genomic structural variation and clarifying the evolution of polyploid cotton. A total of 36956 SSRs, including 11289 released in previous studies and 25567 which were newly developed based on the genome sequences of G. arboreum and G. raimondii, were utilized to construct a new genetic map. The new high-density genetic map includes 6009 loci and spanned 3863.97 cM with an average distance of 0.64 cM between consecutive markers. Four inversions (one between Chr08 and Chr24, one between Chr09 and Chr23 and two between Chr10 and Chr20) were identified by homology analysis. Comparative genomic analysis between genetic map and two diploid cottons showed that structural variations between the A genome and At subgenome are more extensive than between D genome and Dt subgenome. A total of 17 inversions, seven simple translocations and two reciprocal translocations were identified between genetic map and G. raimondii. Good colinearity was revealed between the corresponding chromosomes of tetraploid G. hirsutum and G. barbadense genomes, but a total of 16 inversions were detected between them. These results will accelerate the process of evolution analysis of Gossipium genus.

【 授权许可】

Unknown