Minireview: Novel Micropeptide Discovery by Proteomics and Deep Sequencing Methods

Frontiers in Genetics

Akira Sawa¹  Ravi Tharakan³ 

[1] Department of Mental Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States;Departments of Psychiatry, Neuroscience, Biomedical Engineering, and Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States;National Institute on Aging, National Institutes of Health, Baltimore, MD, United States;

关键词: micropeptides;    miniproteins;    proteogenomics;    sORF;    ribosome profiling;    proteomics;   

DOI  :  10.3389/fgene.2021.651485

来源: DOAJ

【 摘 要 】

A novel class of small proteins, called micropeptides, has recently been discovered in the genome. These proteins, which have been found to play important roles in many physiological and cellular systems, are shorter than 100 amino acids and were overlooked during previous genome annotations. Discovery and characterization of more micropeptides has been ongoing, often using -omics methods such as proteomics, RNA sequencing, and ribosome profiling. In this review, we survey the recent advances in the micropeptides field and describe the methodological and conceptual challenges facing future micropeptide endeavors.

【 授权许可】

Unknown