Bead-based assays to simultaneously detect multiple human inherited blood disorders associated with malaria

Malaria Journal

Sodiomon B. Sirima¹  Alfred B. Tiono¹  Alphonse Ouédraogo¹  Edith C. Bougouma¹  Issa Nébié¹  Sam A. Coulibaly¹  Guillaume S. Sanou¹  Lynn Grignard²  Catherine Mair²  Bronner P. Gonçalves²  Chris Drakeley²  Teun Bousema²  Kjerstin H. W. Lanke³  Guide J. H. Bastiaens³  Susana Campino⁴  Taane G. Clark⁴  Muna Affara⁵  Joseph Okebe⁵  Umberto d’Alessandro⁵  Jonathan Curry⁶  Laleta Mahey⁶ 

[1] Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme;Department of Immunology and Infection, London School of Hygiene & Tropical Medicine;Department of Medical Microbiology, Radboud University Medical Centre;Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine;Disease Control & Elimination Theme, Medical Research Council Unit at London School of Hygiene and Tropical Medicine;LGC Genomics;

关键词: Malaria;    Glucose-6-phosphate dehydrogenase deficiency;    Haemoglobin S;    Haemoglobin C;    Multiplex detection;   

DOI  :  10.1186/s12936-019-2648-7

来源: DOAJ

【 摘 要 】

Abstract Background Glucose-6-phosphate dehydrogenase deficiency (G6PDd), haemoglobin C (HbC) and S (HbS) are inherited blood disorders (IBD) common in populations in malaria endemic areas. All are associated to some degree with protection against clinical malaria whilst additionally G6PDd is associated with haemolysis following treatment with 8-aminoquinolines. Measuring the prevalence of these inherited blood disorders in affected populations can improve understanding of disease epidemiology. Current methodologies in epidemiological studies commonly rely on individual target amplification and visualization; here a method is presented to simultaneously detect the polymorphisms and that can be expanded to include other single nucleotide polymorphisms (SNPs) of interest. Methods Human DNA from whole blood samples was amplified in a novel, multiplex PCR reaction and extended with SNP-specific probes in an allele specific primer extension (ASPE) to simultaneously detect four epidemiologically important human markers including G6PD SNPs (G202A and A376G) and common haemoglobin mutations (HbS and HbC). The products were hybridized to magnetic beads and the median fluorescence intensity (MFI) was read on MAGPIX® (Luminex corp.). Genotyping data was compared to phenotypical data generated by flow cytometry and to established genotyping methods. Results Seventy-five samples from Burkina Faso (n = 75/78, 96.2%) and 58 samples from The Gambia (n = 58/61, 95.1%) had a G6PD and a HBB genotype successfully assigned by the bead-based assay. Flow cytometry data available for n = 61 samples further supported the concordance between % G6PD normal/deficient cells and genotype. Conclusions The bead based assay compares well to alternative measures of genotyping and phenotyping for G6PD. The screening is high throughput, adaptable to inclusion of multiple targets of interest and easily standardized.

【 授权许可】

Unknown