Molecular Alterations in Dog Pheochromocytomas and Paragangliomas

Cancers

ClaudiaE. Reusch¹  Annelies de Klein²  Bert Eussen²  Sara Galac³  GuyC. M. Grinwis⁴  RonaldR. de Krijger⁵  DaphneA. E. R. Dieduksman⁶  NielsM. G. Krol⁶  Hans Stoop⁶  WinandN. M. Dinjens⁶  Esther Korpershoek⁶  Federico Fracassi⁷  AndréG. Uitterlinden⁸  Monika Hilbe⁹  MichaelJ. Day^1,10 

[1] Clinic for Small Animal Internal Medicine, University of Zurich, 8057 Zurich, Switzerland;Department of Clinical Genetics, Erasmus MC University Medical Center, 3015 GD Rotterdam, The Netherlands;Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands;Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands;Department of Pathology University Medical Center/Princess Maxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands;Department of Pathology, Erasmus MC Cancer Institute, University Medical Center, 3015 GD Rotterdam, The Netherlands;Department of Veterinary Medical Science, University of Bologna, 40064 Ozzano dell’Emilia, Italy;Genetic Laboratory, Erasmus MC University Medical Center, 3015 GD Rotterdam, The Netherlands;Institute of Veterinary Pathology, University of Zurich, 8057 Zurich, Switzerland;School of Veterinary and Life Sciences, Murdoch University, Murdoch 6150, WA, Australia;

关键词: dog;    pheochromocytoma;    paraganglioma;    SDHB;    SDHD;    mutation;    chromosomal alteration;    comparative genomics;   

DOI  :  10.3390/cancers11050607

来源: DOAJ

【 摘 要 】

Recently, genetic alterations in the genes encoding succinate dehydrogenase subunit B and D (SDHB and SDHD) were identified in pet dogs that presented with spontaneously arising pheochromocytomas (PCC) and paragangliomas (PGL; together PPGL), suggesting dogs might be an interesting comparative model for the study of human PPGL. To study whether canine PPGL resembled human PPGL, we investigated a series of 50 canine PPGLs by immunohistochemistry to determine the expression of synaptophysin (SYP), tyrosine hydroxylase (TH) and succinate dehydrogenase subunit A (SDHA) and B (SDHB). In parallel, 25 canine PPGLs were screened for mutations in SDHB and SDHD by Sanger sequencing. To detect large chromosomal alterations, single nucleotide polymorphism (SNP) arrays were performed for 11 PPGLs, including cases for which fresh frozen tissue was available. The immunohistochemical markers stained positive in the majority of canine PPGLs. Genetic screening of the canine tumors revealed the previously described variants in four cases; SDHB p.Arg38Gln (n = 1) and SDHD p.Lys122Arg (n = 3). Furthermore, the SNP arrays revealed large chromosomal alterations of which the loss of chromosome 5, partly homologous to human chromosome 1p and chromosome 11, was the most frequent finding (100% of the six cases with chromosomal alterations). In conclusion, canine and human PPGLs show similar genomic alterations, suggestive of common interspecies PPGL-related pathways.

【 授权许可】

Unknown