Digital PCR: What Relevance to Plant Studies?

Biology

Paola Carnevali¹  Chiara Delogu²  Lorella Andreani²  Valeria Terzi³  Roberta Ghizzoni³  Caterina Morcia³ 

[1] Barilla S.p.A., Via Mantova 166, I-43122 Parma, Italy;Consiglio per la Ricerca in Agricoltura e L’analisi Dell’economia Agraria-Centro di Ricerca Difesa e Certificazione (CREA-DC), Via Emilia km 307, 26838-Tavazzano, Italy;Consiglio per la Ricerca in Agricoltura e L’analisi Dell’economia Agraria-Centro di Ricerca Genomica e Bioinformatica (CREA-GB), Via San Protaso 302, 29017-Fiorenzuola d’Arda, Italy;

关键词: digital PCR;    genetic traceability;    diagnostics;    genetically modified organisms;    species;    copy number variation;   

DOI  :  10.3390/biology9120433

来源: DOAJ

【 摘 要 】

Digital PCR (dPCR) is a breakthrough technology that able to provide sensitive and absolute nucleic acid quantification. It is a third-generation technology in the field of nucleic acid amplification. A unique feature of the technique is that of dividing the sample into numerous separate compartments, in each of which an independent amplification reaction takes place. Several instrumental platforms have been developed for this purpose, and different statistical approaches are available for reading the digital output data. The dPCR assays developed so far in the plant science sector were identified in the literature, and the major applications, advantages, disadvantages, and applicative perspectives of the technique are presented and discussed in this review.

【 授权许可】

Unknown