Dietary Intervention, When Not Associated With Exercise, Upregulates Irisin/FNDC5 While Reducing Visceral Adiposity Markers in Obese Rats

Frontiers in Physiology

Iran Malavazi¹  Ricardo Carneiro Borra¹  Marina Campos Rocha¹  Cynthia Aparecida de Castro²  Diego Adorna Marine³  Ana Cláudia Garcia de Oliveira Duarte³  Marcela Sene-Fiorese³  Vanessa de Oliveira Furino³  Carla Nascimento dos Santos Rodrigues³  João Manoel Alves³  Stela Márcia Mattiello⁴  Cristina Arrais-Lima⁴ 

[1] Department of Genetics and Evolution-Biological and Health Sciences Center – CCBS, Federal University of São Carlos – UFSCar, São Carlos, Brazil;Department of Morphology and Pathology-Biological and Health Sciences Center – CCBS, Federal University of São Carlos – UFSCar, São Carlos, Brazil;Department of Physical Education and Human Motricity – DEFMH, Biological and Health Sciences Center – CCBS, Federal University of São Carlos – UFSCar, São Carlos, Brazil;Department of Physiotherapy – DFisio-Biological and Health Sciences Center – CCBS, Federal University of São Carlos – UFSCar, São Carlos, Brazil;

关键词: body composition;    endurance training;    visceral adipose tissue;    high-fat diet;    obesity;    irisin/FNDC5;   

DOI  :  10.3389/fphys.2021.564963

来源: DOAJ

【 摘 要 】

Obesity is an epidemic disease and the expansion of adipose tissue, especially visceral fat, promotes the secretion of factors that lead to comorbidities such as diabetes and cardiovascular diseases. Thus, diet and exercise have been proposed as an intervention to reverse these complications. An adipocytokine, known as irisin, mediates the beneficial effects of exercise. It has been proposed as a therapeutic potential in controlling obesity. In view of the above, this paper attempts to determine the modulation of irisin, visceral adiposity and biochemical markers in response to dietary intervention and aerobic exercise. To do this, 52 diet-induced obese male Wistar rats were divided into the following four groups: high-fat diet and exercise (HFD-Ex); HFD-Sedentary (HFD-Sed); chow-diet and exercise (CD-Exercise); and CD-Sed. The exercise-trained group performed a treadmill protocol for 60 min/day, 3 days/week for 8 weeks. Body mass (BM), body fat (BF), fat mass (FM), and fat-free mass (FFM) were analyzed. Mesenteric (MES), epididymal (EPI), and retroperitoneal (RET) adipose tissue was collected and histological analysis was performed. Biochemical irisin, triglycerides, glucose, insulin and inflammatory markers were determined and, FNDC5 protein expression was analyzed. In this study, the diet was the most important factor in reducing visceral adiposity in the short and long term. Exercise was an important factor in preserving muscle mass and reducing visceral depots after a long term. Moreover, the combination of diet and exercise can enhance these effects. Diet and exercise exclusively were the factors capable of increasing the values of irisin/FNDC5, however it did not bring cumulative effects of both interventions. Prescriptions to enhance the obesity treatments should involve reducing visceral adiposity by reducing the fat content in the diet associated with aerobic exercise.

【 授权许可】

Unknown