Influence of Gut Microbiota on Progression to Tuberculosis Generated by High Fat Diet-Induced Obesity in C3HeB/FeJ Mice

Frontiers in Immunology

Iñaki Comas²  Paula Cardona³  Pere-Joan Cardona³  Lilibeth Arias³  Cristina Vilaplana³  Eric Garcia⁴  Jorge Díaz⁴  Yaiza Rosales⁴  Gustavo Tapia⁵  Manuela Torres-Puente⁶  Galo Adrián Goig⁶ 

[1] Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Madrid, Spain;Centro de Investigación Biomédica en Red de Epidemiologia y Salud Pública (CIBERESP), Madrid, Spain;Department of Genetics and Microbiology, Universitat Autònoma de Barcelona (UAB), Badalona, Spain;Experimental Tuberculosis Unit (UTE), Fundació Institut Germans Trias i Pujol (IGTP), Badalona, Spain;Pathology Department, Hospital Universitari Germans Trias i Pujol (HUGTIP), Universitat Autònoma de Barcelona (UAB), Badalona, Spain;Tuberculosis Genomics Unit (TGU), Instituto de Biomedicina de Valencia (IBV-CSIC), Valencia, Spain;

关键词: tuberculosis;    high fat diet;    obesity;    comorbidity;    BCG;    mice;   

DOI  :  10.3389/fimmu.2019.02464

来源: DOAJ

【 摘 要 】

The administration of a high fat content diet is an accelerating factor for metabolic syndrome, impaired glucose tolerance, and early type 2 diabetes. The present study aims to assess the impact of a high fat diet on tuberculosis progression and microbiota composition in an experimental animal model using a C3HeB/FeJ mouse strain submitted to single or multiple consecutive aerosol infections. These models allowed us to study the protection induced by Bacillus Calmette-Guérin vaccination as well as by the natural immunity induced by chemotherapy after a low dose Mycobacterium tuberculosis infection. Our results show that a high fat diet is able to trigger a pro-inflammatory response, which results in a faster progression toward active tuberculosis and an impaired protective effect of BCG vaccination, which is not the case for natural immunity. This may be related to dysbiosis and a reduction in the Firmicutes/Bacteroidetes ratio in the gut microbiota caused by a decrease in the abundance of the Porphyromonadaceae family and, in particular, the Barnesiella genus. It should also be noted that a high fat diet is also related to an increase in the genera Alistipes, Parasuterella, Mucispirillum, and Akkermansia, which have previously been related to dysbiotic processes. As diabetes mellitus type 2 is a risk factor for developing tuberculosis, these findings may prove useful in the search for new prophylactic strategies for this population subset.

【 授权许可】

Unknown