Leguminous Lectins as Tools for Studying the Role of Sugar Residues in Leukocyte Recruitment

Mediators of Inflammation

B. S. Cavada¹  E. H. Teixeira¹  N. M. N. Alencar²  A. M. S. Assreuy³  R. A. Ribeiro³  C. A. Flores³ 

[1] Department of Biochemistry and Molecular Biology, Federal University of Ceara, PO Box 3157, Rua Coronel Nunes de Melo 1127, Fortaleza, CE 60430.270, Brazil;Department of Clinical and Toxicological Analysis, Federal University of Ceara, PO Box 3157, Rua Coronel Nunes de Melo 1127, Fortaleza, CE 60430.270, Brazil;Department of Physiology and Pharmacology, Federal University of Ceara, PO Box 3157, Rua Coronel Nunes de Melo 1127, Fortaleza, CE 60430.270, Brazil;

关键词: Anti-inflammatory;    Leguminous lectins;    Neutrophil migration;    Rat paw oedema;    Sugar residues.;   

DOI  :  10.1080/09629359990603

来源: DOAJ

【 摘 要 】

The natural physiological ligands for selectins are oligosaccharides found in glycoprotein or glycolipid molecules in cell membranes. In order to study the role of sugar residues in the in vivo lectin anti-inflammatory effect, we tested three leguminous lectins with different carbohydrate binding affinities in the peritonitis and paw oedema models induced by carrageenin in rats. L. sericeus lectin was more anti-inflammatory than D. virgata lectin, the effects being reversed by their specific binding sugars (N-acetylglucosamine and α-methylmannoside, respectively). However, V. macrocarpa, a galactose-specific lectin, was not anti-inflammatory. The proposed anti-inflammatory activity of lectins could be due to a blockage of neutrophil-selectin carbohydrate ligands. Thus, according to the present data, we suggest an important role for N-acetylglucosamine residue as the major ligand for selectins on rat neutrophil membranes.

【 授权许可】

Unknown