μECoG Recordings Through a Thinned Skull

Frontiers in Neuroscience

Sanitta Thongpang¹  Kevin P. Cheng²  Sarah K. Brodnick²  Thomas J. Richner²  Jared P. Ness²  Mohammed Hayat²  Joseph Novello²  Justin C. Williams³  Aaron J. Suminski³  Kip A. Ludwig³  Lisa Krugner-Higby⁴ 

[1] Department of Biomedical Engineering, Mahidol University, Salaya, Thailand;Department of Biomedical Engineering, University of Wisconsin–Madison, Madison, WI, United States;Department of Neurological Surgery, University of Wisconsin–Madison, Madison, WI, United States;Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin–Madison, Madison, WI, United States;

关键词: thinned skull;    μECoG;    local field potenitals;    optogenetics;    somatosensory evoked potentials;   

DOI  :  10.3389/fnins.2019.01017

来源: DOAJ

【 摘 要 】

The studies described in this paper for the first time characterize the acute and chronic performance of optically transparent thin-film micro-electrocorticography (μECoG) grids implanted on a thinned skull as both an electrophysiological complement to existing thinned skull preparation for optical recordings/manipulations, and a less invasive alternative to epidural or subdurally placed μECoG arrays. In a longitudinal chronic study, μECoG grids placed on top of a thinned skull maintain impedances comparable to epidurally placed μECoG grids that are stable for periods of at least 1 month. Optogenetic activation of cortex is also reliably demonstrated through the optically transparent μECoG grids acutely placed on the thinned skull. Finally, spatially distinct electrophysiological recordings were evident on μECoG electrodes placed on a thinned skull separated by 500–750 μm, as assessed by stimulation evoked responses using optogenetic activation of cortex as well as invasive and epidermal stimulation of the sciatic and median nerve at chronic time points. Neural signals were collected through a thinned skull in mice and rats, demonstrating potential utility in neuroscience research applications such as in vivo imaging and optogenetics.

【 授权许可】

Unknown