Identification of the Valid Reference Genes for QuantitativeRT-PCR in Annual Ryegrass (Lolium multiflorum) underSalt Stress

Molecules

Linkai Huang¹  Xinquan Zhang¹  Xia Wang¹  Xiao Ma¹ 

[1] Grassland Science Department, Sichuan Agriculture University, Chengdu 611130, China;

关键词: annual ryegrass;    qRT-PCR;    reference gene;    salt stress;   

DOI  :  10.3390/molecules20034833

来源: DOAJ

【 摘 要 】

Annual ryegrass (Lolium multiflorum) is a cool-season annual grass cultivated worldwide for its high yield and quality. With the areas of saline soil increasing, investigation of the molecular mechanisms of annual ryegrass tolerance under salt stress has become a significant topic. qRT-PCR has been a predominant assay for determination of the gene expression, in which selecting a valid internal reference gene is a crucial step.The objective of present study was to evaluate and identify suitable reference genes forqRT-PCR in annual ryegrass under salt stress. The results calculated by RefFinder indicated that eEF1A(s) was the most stable reference gene in leaves, whereas EF1-a was the least stable; meanwhile, TBP-1 was the most optimal in roots and in all samples, and the eIF-5A shouldn’t be utilized for normalization of the gene expression. eEF1A(s) is more suitable than TBP-1 as reference gene in leaves when verified with P5CS1 andCyt-Cu/Zn SOD genes. We should choose optimal reference genes in specific tissues instead of the most stable one selected from different conditions and tissues.

【 授权许可】

Unknown