Expression, divergence and evolution of the caleosin gene family in Brassica rapa

Archives of Biological Sciences

关键词: Brassica rapa;    caleosin;    functional divergence;    evolution;    expression;   

DOI  :  10.2298/ABS1303863H

来源: DOAJ

【 摘 要 】

Caleosins (CLO) are oil body-associated proteins encoded by a small genefamily. To investigate the expression, functional diversity and evolutionarymodes of CLO genes, we isolated and integrally analyzed in silico a total of11 CLO genes from Brassica rapa. According to phylogeny and sequenceanalyses, 11 BrCLO genes were classified into 3 groups, and each group sharedhighly conserved sequence features. Syntenic analysis revealed that allmembers of the BrCLO gene family distributed on 7 chromosomes were expandedmainly by segmental duplications. Evolutionary analysis showed that CLOproteins were controlled by purifying selection in B. rapa. Interestingly,functional divergence studies indicated that site-specific relaxed functionalconstraints were present between the different clusters of caleosins.Expression pattern suggested that 6 BrCLO genes were potentially associatedwith oil body formation. Our findings provide valuable clues for aninvestigation of the evolutionary history and cellular functions of the CLOgene family in plants.

【 授权许可】

Unknown