Mulberry EIL3 confers salt and drought tolerances and modulates ethylene biosynthetic gene expression

PeerJ

Jun Li¹  Changying Liu²  Dingpei Long²  Panpan Zhu²  Aichun Zhao²  Jian Yu²  Jiamin Hou²  Maode Yu²  Chuanhong Wang³ 

[1] Guiyang University of Chinese Medicine, Guiyang, China;State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Chongqing, China;The National Key Engineering Lab of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei, China;

关键词: MnEIL3;    Mulberry;    Arabidopsis;    Abiotic stresses;    Salt stress;    Drought;   

DOI  :  10.7717/peerj.6391

来源: DOAJ

【 摘 要 】

Ethylene regulates plant abiotic stress responses and tolerances, and ethylene-insensitive3 (EIN3)/EIN3-like (EIL) proteins are the key components of ethylene signal transduction. Although the functions of EIN3/EIL proteins in response to abiotic stresses have been investigated in model plants, little is known in non-model plants, including mulberry (Morus L.), which is an economically important perennial woody plant. We functionally characterized a gene encoding an EIN3-like protein from mulberry, designated as MnEIL3. A quantitative real-time PCR analysis demonstrated that the expression of MnEIL3 could be induced in roots and shoot by salt and drought stresses. Arabidopsis overexpressing MnEIL3 exhibited an enhanced tolerance to salt and drought stresses. MnEIL3 overexpression in Arabidopsis significantly upregulated the transcript abundances of ethylene biosynthetic genes. Furthermore, MnEIL3 enhanced the activities of the MnACO1 and MnACS1 promoters, which respond to salt and drought stresses. Thus, MnEIL3 may play important roles in tolerance to abiotic stresses and the expression of ethylene biosynthetic genes.

【 授权许可】

Unknown