Transglutaminase 2 Has Metabolic and Vascular Regulatory Functions Revealed by In Vivo Activation of Alpha1-Adrenergic Receptor

International Journal of Molecular Sciences

Kinga Lénárt¹  András Mádi¹  László Fésüs¹  Attila Pap¹  Anna V. Oláh²  Róbert Pórszász³ 

[1] Department of Biochemistry and Molecular Biology, University of Debrecen, H-4032 Debrecen, Hungary;Department of Laboratory Medicine, University of Debrecen, H-4032 Debrecen, Hungary;Department of Pharmacology and Pharmacotherapy, University of Debrecen, H-4032 Debrecen, Hungary;

关键词: transglutaminase 2 KO;    CLAMS;    respiratory exchange ratio;    heart failure;    lactate dehydrogenase;    blood pressure;   

DOI  :  10.3390/ijms21113865

来源: DOAJ

【 摘 要 】

The multifunctional tissue transglutaminase has been demonstrated to act as α1-adrenergic receptor-coupled G protein with GTPase activity in several cell types. To explore further the pathophysiological significance of this function we investigated the in vivo effects of the α1-adrenergic receptor agonist phenylephrine comparing responses in wild type and TG2^-/- mice. Injection of phenylephrine, but not a beta3-adrenergic agonist (CL-316,243), resulted in the long-term decline of the respiratory exchange ratio and lower lactate concentration in TG2^-/- mice indicating they preferred to utilize fatty acids instead of glucose as fuels. Measurement of tail blood pressure revealed that the vasoconstrictive effect of phenylephrine was milder in TG2^-/- mice leading to lower levels of lactate dehydrogenase (LDH) isoenzymes in blood. LDH isoenzyme patterns indicated more damage in lung, liver, kidney, skeletal, and cardiac muscle of wild type mice; the latter was confirmed by a higher level of heart-specific CK-MB. Our data suggest that TG2 as an α1-adrenergic receptor-coupled G protein has important regulatory functions in alpha1-adrenergic receptor-mediated metabolic processes and vascular functions.

【 授权许可】

Unknown