Potential Biomedical Applications of Collagen Filaments derived from the Marine Demosponges Ircinia oros (Schmidt, 1864) and Sarcotragus foetidus (Schmidt, 1862)

Marine Drugs

Andrea Dodero¹  Silvia Vicini¹  Maila Castellano¹  Dario Cavallo¹  Sara Ferrando²  Marco Giovine²  Eleonora Tassara²  Stefano Aicardi²  Marina Pozzolini²  Marco Bertolino²  Iaroslav Petrenko³  Hermann Ehrlich³ 

[1] Department of Chemistry and Industrial Chemistry (DCCI), University of Genova, Via Dodecaneso 31, 16146 Genova, Italy;Department of Earth, Environment and Life Sciences (DISTAV), University of Genova, Via Pastore 3, 16132 Genova, Italy;Institute of Electronic and Sensor Materials, TU Bergakademie Freiberg, 09599 Freiberg, Germany;

关键词: porifera;    demosponges;    biomaterial;    collagen;    spongin;    wound healing;   

DOI  :  10.3390/md19100563

来源: DOAJ

【 摘 要 】

Collagen filaments derived from the two marine demosponges Ircinia oros and Sarcotragus foetidus were for the first time isolated, biochemically characterised and tested for their potential use in regenerative medicine. SDS-PAGE of isolated filaments revealed a main collagen subunit band of 130 kDa in both of the samples under study. DSC analysis on 2D membranes produced with collagenous sponge filaments showed higher thermal stability than commercial mammalian-derived collagen membranes. Dynamic mechanical and thermal analysis attested that the membranes obtained from filaments of S. foetidus were more resistant and stable at the rising temperature, compared to the ones derived from filaments of I. oros. Moreover, the former has higher stability in saline and in collagenase solutions and evident antioxidant activity. Conversely, their water binding capacity results were lower than that of membranes obtained from I. oros. Adhesion and proliferation tests using L929 fibroblasts and HaCaT keratinocytes resulted in a remarkable biocompatibility of both developed membrane models, and gene expression analysis showed an evident up-regulation of ECM-related genes. Finally, membranes from I. oros significantly increased type I collagen gene expression and its release in the culture medium. The findings here reported strongly suggest the biotechnological potential of these collagenous structures of poriferan origin as scaffolds for wound healing.

【 授权许可】

Unknown