Polymorphisms of Codons 110, 146, 211 and 222 at the Goat PRNP Locus and Their Association with Scrapie in Greece

Animals

Athanasios I. Gelasakis¹  Jan P. M. Langeveld²  Vayia Palaska³  Dimitrios Loukovitis⁴  Nektarios D. Giadinis⁵  Dimitra Papakostaki⁶  Efstathios Katharopoulos⁷  Evridiki Boukouvala⁷  Loukia V. Ekateriniadou⁷  Maria Babetsa⁷ 

[1] Department of Animal Science, School of Animal Biosciences, Agricultural University of Athens, 11855 Athens, Greece;Department of Infection Biology, Wageningen Bioveterinary Research (WBVR), 8221 RA Lelystad, The Netherlands;National Reference Laboratory for TSEs, Ministry of Agricultural Development and Food, 41110 Larissa, Greece;Research Institute of Animal Science, ELGO-DIMITRA, 58100 Giannitsa, Greece;School of Veterinary Medicine, Aristotle University of Thessaloniki, 54627 Thessaloniki, Greece;Veterinary Center of Thessaloniki, Ministry of Agricultural Development and Food, 54627 Thessaloniki, Greece;Veterinary Research Institute, ELGO-DIMITRA, 54124 Thessaloniki, Greece;

关键词: scrapie;    goats;    polymorphisms;    genetic resistance;    breeding strategies;   

DOI  :  10.3390/ani11082340

来源: DOAJ

【 摘 要 】

Scrapie is considered an endemic disease in both sheep and goats in Greece. However, contrary to sheep, in goats more than one prion protein (PrP) polymorphism has been recognized as a candidate for resistance breeding against the disease. For an impression, candidates which are circulating, (i) brain samples (n = 525) from scrapie-affected (n = 282) and non-affected (n = 243) animals within the national surveillance program, and (ii) individual blood samples (n = 1708) from affected (n = 241) and non-affected (n = 1467) herds, in a large part of mainland Greece and its islands, were collected and assayed. A dedicated Taqman method was used to test for amino acid polymorphisms 110T/P, 146N/S/D, 211R/Q, and 222Q/K. Highly prevalent genotypes were 110TT, 146NN, 211RR, and 222QQ. The frequencies of polymorphisms in blood and negative brain samples for codons 110P, 211Q, and 222K were 4.0%, 3.0%, and 1.9%, respectively, while 146D (0.7%) was present only on Karpathos island. Codon 110P was exclusively found in scrapie-negative brains, and homozygous 110P/P in two scrapie-negative goats. It is concluded that breeding programs in Karpathos could focus on codon 146D, while in other regions carriers of the 110P and 222K allele should be sought. Case-control and challenge studies are now necessary to elucidate the most efficient breeding strategies.

【 授权许可】

Unknown