Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly

eLife

Toshiyuki Oda¹  Masahide Kikkawa²  Hiroshi Yamaguchi³  Hiroyuki Takeda³ 

[1] Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan;Department of Anatomy and Structural Biology, Graduate School of Medicine, University of Yamanashi, Yamanashi, Japan;Department of Cell Biology and Anatomy, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan;

关键词: cilia;    axonemal dynein;    cryo-electron tomography;    sperm;    zebrafish;    PIH protein;   

DOI  :  10.7554/eLife.36979

来源: DOAJ

【 摘 要 】

Construction of motile cilia/flagella requires cytoplasmic preassembly of axonemal dyneins before transport into cilia. Axonemal dyneins have various subtypes, but the roles of each dynein subtype and their assembly processes remain elusive in vertebrates. The PIH protein family, consisting of four members, has been implicated in the assembly of different dynein subtypes, although evidence for this idea is sparse. Here, we established zebrafish mutants of all four PIH-protein genes: pih1d1, pih1d2, ktu, and twister, and analyzed the structures of axonemal dyneins in mutant spermatozoa by cryo-electron tomography. Mutations caused the loss of specific dynein subtypes, which was correlated with abnormal sperm motility. We also found organ-specific compositions of dynein subtypes, which could explain the severe motility defects of mutant Kupffer’s vesicle cilia. Our data demonstrate that all vertebrate PIH proteins are differently required for cilia/flagella motions and the assembly of axonemal dyneins, assigning specific dynein subtypes to each PIH protein.

【 授权许可】

Unknown