Rice Potassium Transporter OsHAK8 Mediates K+ Uptake and Translocation in Response to Low K+ Stress

Frontiers in Plant Science

Sheng Luan¹  Fei Li²  Dan Cai²  Junfeng Li²  Yu Pan²  Liangbi Chen²  Dandan Mao²  Xiaohui Wang² 

[1] Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United States;Hunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Sciences, Hunan Normal University, Changsha, China;

关键词: potassium;    rice;    OsHAK8;    K+ uptake;    K+ translocation;    low K+ stress;   

DOI  :  10.3389/fpls.2021.730002

来源: DOAJ

【 摘 要 】

Potassium (K+) levels in the soil often limit plant growth and development. As a result, crop production largely relies on the heavy use of chemical fertilizers, presenting a challenging problem in sustainable agriculture. To breed crops with higher K+-use efficiency (KUE), we must learn how K+ is acquired from the soil by the root system and transported to the rest of the plant through K+ transporters. In this study, we identified the function of the rice K+ transporter OsHAK8, whose expression level is downregulated in response to low-K+ stress. When OsHAK8 was disrupted by CRISPR/Cas9-mediated mutagenesis, Oshak8 mutant plants showed stunted growth, especially under low-K+ conditions. Ion content analyses indicated that K+ uptake and root-to-shoot K+ transport were significantly impaired in Oshak8 mutants under low-K+ conditions. As the OsHAK8 gene was broadly expressed in different cell types in the roots and its protein was targeted to the plasma membrane, we propose that OsHAK8 serves as a major transporter for both uptake and root-to-shoot translocation in rice plants.

【 授权许可】

Unknown