Proximity assays for sensitive quantification of proteins

Biomolecular Detection and Quantification

David Ruff¹  Gemma Johnson²  Sara Kirvell²  Christina Greenwood²  Harvinder S. Dhillon²  Stephen A. Bustin² 

[1] Fluidigm Corporation, South San Francisco, CA 94080, USA;Postgraduate Medical Institute, Faculty of Medical Science, Anglia Ruskin University, Chelmsford, Essex CM1 1SQ, UK;

关键词: Immunoassays;    Immuno-PCR;    Proximity ligation assay;    Proximity extension assay;    In situ assays;   

DOI  :  10.1016/j.bdq.2015.04.002

来源: DOAJ

【 摘 要 】

Proximity assays are immunohistochemical tools that utilise two or more DNA-tagged aptamers or antibodies binding in close proximity to the same protein or protein complex. Amplification by PCR or isothermal methods and hybridisation of a labelled probe to its DNA target generates a signal that enables sensitive and robust detection of proteins, protein modifications or protein–protein interactions. Assays can be carried out in homogeneous or solid phase formats and in situ assays can visualise single protein molecules or complexes with high spatial accuracy. These properties highlight the potential of proximity assays in research, diagnostic, pharmacological and many other applications that require sensitive, specific and accurate assessments of protein expression.

【 授权许可】

Unknown