Point-of-need molecular processing of biosamples using portable instrumentation to reduce turnaround time

Biosafety and Health

Yiannis Manoussopoulos¹  Stavroula Siamoglou²  Aristea Velegraki³  Stamatis Vassilakis³  Zoe Kordou⁴  Manousos E. Kambouris⁴  Aphroditi Milioni⁴  Stavroula Goudoudaki⁴  George P. Patrinos⁵  Stavroula Kritikou⁵ 

[1] Amerikis, Patras 26004, Greece;Corresponding author: Laboratory of Pharmacogenomics and Personalized Medicine, Department of Pharmacy, University of Patras, Rio Patras 26504, Greece.;Department of Microbiology, School of Medicine, National and Kapodistrian University of Athens, Mikras Asias 75, Athens 11527, Greece;Department of Pharmacy, University of Patras, Rio Patras 26504, Greece;;Plant Protection Division of Patras, Institute of Industrial and Forage Plants, N.E.O &

关键词: Point-of-Need;    Biosurveillance;    Public health;    Biosecurity – biosafety;    Portable PCR;    Portable NGS;   

DOI  :  

来源: DOAJ

【 摘 要 】

Infectious agents, both standard and emerging or engineered, might initiate outbreaks with global impact and/or existential- level threat potential. Such agents might infect human, livestock, exploitable plant cultures or the environment at large. Instead of responding by sampling teams which may also carry out prolonged surveillance, a more effective concept is to pre-process the samples, possibly to nucleic acid extract so as to transport them without biological risks by standard channels, or to process them up to a point and return the results, leaving the samples which only need specialized tests and dedicated equipment. In this context a portable device, allowing multiple routine and crisis -management applications due to its inherent flexibility, is scrutinized against alternatives and possible needs so as to develop a novel standard of surveillance and intervention, meant to facilitate containment by providing faster much more reliable laboratory results at a fraction of older generation mobile nucleic acids analyzers. Additional steps, such as agarose in pills and buffers pre-packaged in expendable syringes with pistons locked by improvised safety features may improve the Complexity Level of the whole system so as to make it appealing to intervention/crisis management personnel, while the instrument proper may double as backup for respective benchtop devices.

【 授权许可】

Unknown