Altered metabolism of mothers of young children with Autism Spectrum Disorder: a case control study

BMC Pediatrics

Kathryn Hollowood-Jones¹  Uwe Kruger¹  Joshua Chuah¹  Juergen Hahn¹  Bryan K. Woodruff²  Christine L. Snozek²  Sivapriya Ramamoorthy³  Elena L. Pollard⁴  Devon M. Coleman⁴  James B. Adams⁴  Stepan Melnyk⁵  S. Jill James⁵ 

[1] Department of Biomedical Engineering, Rensselaer Polytechnic Institute;Mayo Clinic;Metabolon;School for Engineering of Matter, Transport and Energy, Arizona State University;University of Arkansas for Medical Sciences;

关键词: Autism;    Metabolic profile;    Fisher discriminant analysis;    Logistic regression;    Metabolomics;    Mothers;   

DOI  :  10.1186/s12887-020-02437-7

来源: DOAJ

【 摘 要 】

Abstract Background Previous research studies have demonstrated abnormalities in the metabolism of mothers of young children with autism. Methods Metabolic analysis was performed on blood samples from 30 mothers of young children with Autism Spectrum Disorder (ASD-M) and from 29 mothers of young typically-developing children (TD-M). Targeted metabolic analysis focusing on the folate one-carbon metabolism (FOCM) and the transsulfuration pathway (TS) as well as broad metabolic analysis were performed. Statistical analysis of the data involved both univariate and multivariate statistical methods. Results Univariate analysis revealed significant differences in 5 metabolites from the folate one-carbon metabolism and the transsulfuration pathway and differences in an additional 48 metabolites identified by broad metabolic analysis, including lower levels of many carnitine-conjugated molecules. Multivariate analysis with leave-one-out cross-validation allowed classification of samples as belonging to one of the two groups of mothers with 93% sensitivity and 97% specificity with five metabolites. Furthermore, each of these five metabolites correlated with 8–15 other metabolites indicating that there are five clusters of correlated metabolites. In fact, all but 5 of the 50 metabolites with the highest area under the receiver operating characteristic curve were associated with the five identified groups. Many of the abnormalities appear linked to low levels of folate, vitamin B12, and carnitine-conjugated molecules. Conclusions Mothers of children with ASD have many significantly different metabolite levels compared to mothers of typically developing children at 2–5 years after birth.

【 授权许可】

Unknown