Fusion pores with low conductance are cation selective

Cell Reports

Manfred Lindau¹  Satyan Sharma¹  Meng Huang²  Shailendra Singh Rathore³  Joan S. Lenz⁴  Joannalyn B. Delacruz⁴ 

[1] Engineering Physics, Cornell University, 142 Sciences Drive, Ithaca, NY 14853, USA;Medical Informatics in Translational Oncology, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany;Nanoscale Cell Biology Group, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany;;School of Applied &

关键词: fusion pore;    ion selectivity;    exocytosis;    transmitter release;    vesicle fusion;    SNARE complex;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Many neurotransmitters are organic ions that carry a net charge, and their release from secretory vesicles is therefore an electrodiffusion process. The selectivity of early exocytotic fusion pores is investigated by combining electrodiffusion theory, measurements of amperometric foot signals from chromaffin cells with anion substitution, and molecular dynamics simulation. The results reveal that very narrow fusion pores are cation selective, but more dilated fusion pores become anion permeable. The transition occurs around a fusion pore conductance of ∼300 pS. The cation selectivity of a narrow fusion pore accelerates the release of positively charged transmitters such as dopamine, noradrenaline, adrenaline, serotonin, and acetylcholine, while glutamate release may require a more dilated fusion pore.

【 授权许可】

Unknown