Expression profile of miRNA-29a in neural cells

Chinese Journal of Contemporary Neurology and Neurosurgery

Min-yu JIAN¹  Ye LI¹  Ru-quan HAN¹  Yu LU¹ 

[1] Department of Anesthesia, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China;

关键词: MicroRNAs;    Astrocytes;    Neurons;    Cerebral cortex;    Polymerase chain reaction;    Cells, cultured;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objective To investigate the expression profile of microRNA(miRNA)-29a in mice neural cells.  Methods Quantitativereal-time polymerase chain reaction (qRT-PCR) was used to analyze miRNA-29aexpression changes of neurons and astrocytes after 3, 7, 15, 21 d of primaryculture in vitro, and cortex tissue of 3, 7, 15, 21 d old mice. Results miRNA-29a expression increased in nervous system withthe growth of mice. Compared with the 3th day, miRNA-29a relative expression onthe 7th, 15th, 21th days in neurons, astrocytes and cortex tissue were obviouslyhigher (P < 0.05 or P < 0.01). At each time point, therelative expression levels of miRNA-29a in cultured astrocytes were 20-40 timeshigher than in cultured neurons (P = 0.000), and levels in cortextissue were about 1/3 that seen in cultured astrocytes (P = 0.000). Conclusions With the growth and development of brain cortex ofmice, miRNA-29a is highly expressed in astrocytes, reflecting its abundant andspecific expression.

 

DOI: 10.3969/j.issn.1672-6731.2015.01.009

【 授权许可】

Unknown