Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts

Journal of Clinical Medicine

Louis Tong¹  AlexLap-Ki Ng²  FranciscaSiu-Yin Wong²  Yashan Bu²  KendrickCo Shih²  TommyChung-Yan Chan²  AmyCheuk-Yin Lo²  Yau-Kei Chan²  SumSum Kwok²  GaryHin-Fai Yam³  Vishal Jhanji³ 

[1] Cornea and External Eye Disease Service, Singapore National Eye Centre, Singapore 168751, Singapore;Department of Ophthalmology, Li Ka Shing Faculty of Medicine, University of Hong Kong and Hong Kong SAR, Hong Kong, China;Department of Ophthalmology, University of Pittsburgh Medical Centre, Pittsburgh, PA 15213, USA;

关键词: Lycium barbarum polysaccharides;    corneal scarring;    human keratocytes;    fibroblast;    TGFβ1;   

DOI  :  10.3390/jcm9113572

来源: DOAJ

【 摘 要 】

(1) Objective: To study the anti-fibrotic effects of Lycium barbarum polysaccharides (LBP) on corneal stromal fibroblasts and assess LBP’s effect on cell viability. (2) Methods: Primary human corneal keratocytes of passage 3 to 6 were used for all experiments. Cells are pretreated with LBP solution for 24 h and then transforming growth factor beta 1 (TGFβ1) for 48 h and collected for experiments. Fibrotic protein analysis was performed using immunofluorescence and Western blot. The effect of LBP on cell viability was assessed using the MTS assay. (3) Results: LBP significantly reduced the expression of fibrotic proteins, including α-SMA and extracellular matrix proteins (collagen type I and III). LBP significantly decreased the viability of myofibroblasts but not the fibroblasts. Conclusions: In this study, LBP was effective in the prevention of fibrosis gene expression. Further studies to assess the underlying mechanism and pharmacological properties will facilitate the formation of a topical LBP solution for in vivo studies.

【 授权许可】

Unknown