Cerebellar abnormalities following hypoxia alone compared to hypoxic–ischemic forebrain injury in the developing rat brain

Neurobiology of Disease

R. Ann Sheldon¹  Zinaida S. Vexler²  Vivi M. Heine³  Ruggero Spadafora⁴  Catherine Verney⁵  David H. Rowitch⁶  Valerie Biran⁷  Donna M. Ferriero⁸ 

[1] Assistance Publique Hôpitaux de Paris, Service de Pédiatrie et Réanimation Néonatales, Hôpital Robert Debré, Paris, France;Corresponding author. Service de Pédiatrie et Réanimation Néonatales, Hôpital Robert Debré, 48 Blvd Sérurier, F-75019 Paris, France. Fax: [phone].;Inserm, U676, AVENIR RO5230HS, Paris, France;PremUP, Paris, France;Université Paris 7, Faculté de Médecine Denis Diderot, Paris, France;Department of Child Neurology, VU University Medical Center, Amsterdam, The Netherlands;Department of Neurology and Pediatrics, University of California San Francisco, San Francisco, CA, USA;Inserm, U676, AVENIR RO5230HS, Paris, France;

关键词: Cerebellum;    Hypoxia;    Ischemia;    Neuronal death;    Purkinje cells;    Granule cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Two-day-old (P2) rat pups were subjected to either a global hypoxia or to electrocoagulation of the right carotid artery followed by 2.5 h hypoxia. Cellular and regional injury in the cerebellum (CB) was studied at 1, 2 and 19 days using immunohistology. Following hypoxia and hypoxia–ischemia, all neuronal populations of the CB were damaged in a subset of Purkinje cells. The decrease in the number of interneurons, as well as the thickness of molecular and granular layers was significant following hypoxia. Diffuse white matter damage, with loss of preoligodendrocytes was more severe following hypoxia than hypoxia–ischemia. Global hypoxia in the rat at P2 produces extensive damage to many cell types in different areas of the CB. The addition of unilateral forebrain ischemia does not increase the severity of these changes. Our data provide insight into the mechanisms of the changes observed in the CB of premature newborns.

【 授权许可】

Unknown