Development and in vivo evaluation of chitosan-gel containing Mitracarpus frigidus methanolic extract for vulvovaginal candidiasis treatment

Biomedicine & Pharmacotherapy

Priscila Faria Pinto¹  Ari Sérgio de Oliveira Lemos²  Elita Scio²  Lara Melo Campos²  Rodrigo Luiz Fabri²  Ângelo Márcio Leite Denadai³  Giuly Cristina Rodrigues de Mello Botti⁴  Vinícius Novaes Rocha⁴  Janildo Ludolf Reis Júnior⁴  Thiago Pereira da Silva⁵  Guilherme Diniz Tavares⁶  Luisa Ferreira da Cruz⁷  Marcelo Gonzaga de Freitas Araújo⁷ 

[1] Corresponding author at: Bioactive Natural Products Laboratory, Department of Biochemistry, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, CEP 36036-900, Brazil.;Bioactive Natural Products Laboratory and Protein Structure and Function Laboratory, Department of Biochemistry, Institute of Biological Sciences, Federal University of Juiz de Fora, Campus, Juiz de Fora, MG, Brazil;Department of Pharmacy, Institute of Life Sciences, Federal University of Juiz de Fora, Campus Governador Valadares, Governor Valadares, MG, Brazil;Department of Veterinary Medicine, Faculty of Medicine, Federal University of Juiz de Fora, Campus, Juiz de Fora, MG, Brazil;Laboratory of Cellular Biology, Department of Biology, Institute of Biological Sciences, Federal University of Juiz de Fora, Campus, Juiz de Fora, MG, Brazil;Laboratory of Nanostructured Systems Development, Department of Pharmaceutical Science, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus, Juiz de Fora, MG, Brazil;Laboratory of Pharmacology, Federal University of São João Del-Rei, Campus Centro Oeste Dona Lindu, Divinópolis, MG, Brazil;

关键词: Vulvovaginal candidiasis;    Antifungal;    Mitracarpus frigidus;    Chitosan;    Rheological tests;   

DOI  :  

来源: DOAJ

【 摘 要 】

Vulvovaginal candidiasis (VVC) is characterized by inflammatory changes in the vaginal mucosa caused by abnormal colonization of Candida species. Traditional topical therapies using reference antifungal drugs usually present several issues and limitations for VVC treatment. Thus, the interest in new vaginal formulations, mainly those based on compounds from natural origin, has been growing over the last years. Methanolic extract from the plant species Mitracarpus frigidus (Willd. Ex Reem Schult.) K. Schum (MFM) has presented potential antifungal activity against C. albicans vaginal infection. Here, we aimed to develop and characterize a gynecological gel formulation based on chitosan containing MFM and to evaluate its anti-C. albicans effectiveness in the treatment of VVC. First, MFM was incorporated into a gel formulation based on chitosan in three final concentrations: 2.5 %, 5.0 %, and 10.0 %. Next, these gel formulations were subjected to stationary and oscillatory rheological tests. Finally, the gel was tested in an experimental VVC model. The rheological tests indicated pseudoplastic fluids, becoming more viscous and elastic with the increase of the extract concentration, indicating intermolecular interactions. Our in vivo analyses demonstrated a great reduction of vulvovaginal fungal burden and infection accompanied with the reduction of mucosal inflammation after MFM chitosan-gel treatment. The present findings open perspectives for the further use of the MFM-chitosan-gel formulation as a therapeutic alternative for VVC treatment.

【 授权许可】

Unknown