Sustained Delivery of Analgesic and Antimicrobial Agents to Knee Joint by Direct Injections of Electrosprayed Multipharmaceutical-Loaded Nano/Microparticles

Polymers

Min-Jhan Li¹  DaveWei-Chih Chen²  Ying-Chao Chou³  Yi-Hsun Yu³  Shih-Jung Liu³  Yung-Heng Hsu³ 

[1] Department of Mechanical Engineering, Chang Gung University, Tao-Yuan 33302, Taiwan;Department of Orthopedic Surgery, Chang Gung Memorial Hospital-Keelung, Keelung 20401, Taiwan;Department of Orthopedic Surgery, Chang Gung Memorial Hospital-Linkou, Tao-Yuan 33305, Taiwan;

关键词: electrospraying;    biodegradable nano/microparticles;    drug delivery;    septic arthritis;    release characteristics;   

DOI  :  10.3390/polym10080890

来源: DOAJ

【 摘 要 】

In this study, we developed biodegradable lidocaine–/vancomycin–/ceftazidime–eluting poly(d,l–lactide–co–glycolide) (PLGA) nano/microparticulate carriers using an electrospraying process, and we evaluated the release behaviors of the carriers in knee joints. To prepare the particles, predetermined weight percentages of PLGA, vancomycin, ceftazidime, and lidocaine were dissolved in solvents. The PLGA/antibiotic/lidocaine solutions were then fed into a syringe for electrospraying. After electrospraying, the morphology of the sprayed nano/microparticles was elucidated by scanning electron microscopy (SEM). The in vitro antibiotic/analgesic release characteristics of the nano/microparticles were studied using high-performance liquid chromatography (HPLC). In addition, drug release to the synovial tissues and fluids was studied in vivo by injecting drug-loaded nano/microparticles into the knee joints of rabbits. The biodegradable electrosprayed nano/microparticles released high concentrations of vancomycin/ceftazidime (well above the minimum inhibition concentration) and lidocaine into the knee joints for more than 2 weeks and for over 3 days, respectively. Such results suggest that electrosprayed biodegradable nano/microcarriers could be used for the long-term local delivery of various pharmaceuticals.

【 授权许可】

Unknown