An Additive Effect of Promoting Thermogenic Gene Expression in Mice Adipose-Derived Stromal Vascular Cells by Combination of Rosiglitazone and CL316,243

International Journal of Molecular Sciences

Tai-Yong Yu¹  Gui-Yan Chu²  Jia-Min Fan²  Xiao Li²  Xin-E Shi²  Tian-Tuan Jiang³  Gong-She Yang³  You-Lei Li³  Xue-Li Zheng³ 

[1] College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China;;Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&

关键词: adipocyte browning;    rosiglitazone;    CL316,243;    beige;    brite;    thermogenic gene;    additive effect;   

DOI  :  10.3390/ijms18051002

来源: DOAJ

【 摘 要 】

It is well-documented that CL316,243 (a β3 agonist) or rosiglitazone (a PPARγ agonist) can induce white adipocyte populations to brown-like adipocytes, thus increasing energy consumption and combating obesity. However, whether there is a combined effect remains unknown. In the present study, stromal vascular cells of inguinal white adipose tissue (iWAT-SVCs for short) from mice were cultured and induced into browning by CL316,243, rosiglitazone, or both. Results showed that a combination of CL316,243 and rosiglitazone significantly upregulated the expression of the core thermogenic gene Ucp1 as well as genes related with mitochondrial function (Cidea, Cox5b, Cox7a1, Cox8b, and Cycs), compared with the treatment of CL316,243 or rosiglitazone alone. Moreover, co-treatment with rosiglitazone could reverse the downregulation of Adiponectin resulting from CL316,243 stimuli alone. Taken together, a combination of rosiglitazone and CL316,243 can produce an additive effect of promoting thermogenic gene expression and an improvement of insulin sensitivity in mouse iWAT-SVCs.

【 授权许可】

Unknown