Characterization of the ceramide moieties of sphingoglycolipids from mouse brain by ESI-MS/MS

Journal of Lipid Research

Carlos Afonso¹  Françoise Fournier¹  Jean-Claude Tabet²  Iuliana Popa²  Jacques Portoukalian³  Benoit Colsch³  Nicole Baumann³ 

[1] Centre National de la Recherche Scientifique Unité Mixte de Recherche 7613, Structure et Fonction de Molecules Bioactives, Universite Pierre et Marie Curie, 75252 Paris cedex 05, France;Institut National de la Santé et de la Recherche Médicale U346, Laboratoire de Dermatologie, Hopital Edouard Herriot, 69003 Lyon, France;Institut National de la Santé et de la Recherche Médicale U495, Laboratoire de Neurochimie, Hopital de la Salpetriere, 75651 Paris cedex 13, France;

关键词: ion trap;    triple quadrupole;    precursor ion scan;    electrospray ionization tandem mass spectrometry;   

DOI  :  

来源: DOAJ

【 摘 要 】

Sphingoglycolipids (SGLs) are cell membrane constituents. As the ceramide structure influences the biological properties of the SGL, we characterized by electrospray ionization tandem mass spectrometry the molecular species of ceramides present in SGL of mouse brain. We report here for the first time the presence in mammalian brain of sphingadienine (d18:2). Sphingenine (d18:1) is present in all SGL species, in contrast to eicosasphingenine (d20:1), which is a constituent of only gangliosides. Sphingadienine is present in galactosylceramide and sulfatides. Free ceramides contain the three types of bases.Thus, there could be two separate pools of free ceramides (d18:1, d18:2 and d20:1, d18:1) as precursors of complex SGL.

【 授权许可】

Unknown