EVOO Promotes a Less Atherogenic Profile Than Sunflower Oil in Smooth Muscle Cells Through the Extracellular Vesicles Secreted by Endothelial Cells

Frontiers in Nutrition

Eduardo Garcia-Fuentes¹  Carolina Gutierrez-Repiso²  Wilfredo Oliva-Olivera²  Lourdes Garrido-Sánchez²  Virginia Mela³  Francisco J. Tinahones³  Rocío Bautista⁵  Josefa Gómez-Maldonado⁶  Flores Martin-Reyes⁷  Cristina Rodríguez-Díaz⁷  Ailec Ho-Plagaro⁷  Concepción Santiago-Fernandez⁷  Mónicas Tome⁸ 

[1] Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, Málaga, Spain;Centro de Investigación Biomédica en Red-Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Málaga, Spain;Facultad de Medicina, Universidad de Málaga, Málaga, Spain;Instituto de Investigación Biomédica de Málaga-IBIMA, Málaga, Spain;Plataforma Andaluza de Bioinformática-Supercomputing and Bioinnovation Center, Universidad de Málaga, Málaga, Spain;Unidad de Genómica y Ultrasecuenciación-Supercomputing and Bioinnovation Center, Universidad de Málaga, Málaga, Spain;Unidad de Gestión Clínica de Aparato Digestivo, Hospital Universitario Virgen de la Victoria, Málaga, Spain;Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Regional Universitario, Málaga, Spain;Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Universitario Virgen de la Victoria, Málaga, Spain;

关键词: miRNAs;    HUVECs;    extra-virgin olive oil;    sunflower oil;    smooth muscle cells (SMCs);    extracellular vesicles (EVs);   

DOI  :  10.3389/fnut.2022.867745

来源: DOAJ

【 摘 要 】

BackgroundLittle is known about the effect of extra virgin olive (EVOO) and sunflower oil (SO) on the composition of extracellular vesicles (EVs) secreted by endothelial cells and the effects of these EVs on smooth muscle cells (SMCs). These cells play an important role in the development of atherosclerosis.MethodsWe evaluated the effects of endothelial cells-derived EVs incubated with triglyceride-rich lipoproteins obtained after a high-fat meal with EVOO (EVOO-EVs) and SO (SO-EVs), on the transcriptomic profile of SMCs.ResultsWe found 41 upregulated and 19 downregulated differentially expressed (DE)-miRNAs in EVOO-EVs. Afterwards, SMCs were incubated with EVOO-EVs and SO-EVs. SMCs incubated with SO-EVs showed a greater number of DE-mRNA involved in pathways related to cancer, focal adhesion, regulation of actin cytoskeleton, and MAPK, toll-like receptor, chemokine and Wnt signaling pathways than in SMCs incubated with EVOO-EVs. These DE-mRNAs were involved in biological processes related to the response to endogenous stimulus, cell motility, regulation of intracellular signal transduction and cell population proliferation.ConclusionEVOO and SO can differently modify the miRNA composition of HUVEC-derived EVs. These EVs can regulate the SMCs transcriptomic profile, with SO-EVs promoting a profile more closely linked to the development of atherosclerosis than EVOO-EVs.

【 授权许可】

Unknown