Divergent Effectiveness of Multispecies Probiotic Preparations on Intestinal Microbiota Structure Depends on Metabolic Properties

Nutrients

Daniela Capobianco¹  Adriana Carino²  Michele Biagioli²  Patrizia Ricci²  Stefano Fiorucci²  Chiara Fiorucci²  Silvia Marchianò²  Eleonora Distrutti³ 

[1]Department of Public Health and Infectious Diseases, University of Rome La Sapienza, 00185 Rome, Italy

[2]Dipartimento di Scienze Biomediche e Chirurgiche, Università di Perugia, 06123 Perugia, Italy

[3]SC di Gastroenterologia ed Epatologia, Azienda Ospedaliera di Perugia, 06100 Perugia, Italy

关键词: probiotics;    inflammatory bowel disease;    animal studies;    macrophage;    regulatory T (Treg) cells;    lactic acid;    short-chain fatty acids (SCFA);    intestinal microbiota;   

DOI  :  10.3390/nu11020325

来源: DOAJ

【 摘 要 】

A growing body of evidence suggests that probiotic functionality is not accurately predicted by their taxonomy. Here, we have set up a study to investigate the effectiveness of two probiotic formulations containing a blend of seven bacterial species in modulating intestinal inflammation in two rodent models of colitis, induced by treating mice with 2,4,6-Trinitrobenzenesulfonic acid (TNBS) or dextran sodium sulfate (DSS). Despite the taxonomy of the bacterial species in the two probiotic formulations being similar, only one preparation (Blend 2-Vivomixx) effectively attenuated the development of colitis in both models. In the TNBS model of colitis, Blend 2 reduced the expression of pro-inflammatory genes while increasing the production of anti-inflammatory cytokines, promoting the expansion M2 macrophages and the formation of IL-10-producing Treg cells in the colon’s lamina propria. In the DSS model of colitis, disease attenuation and Treg formation was observed only in mice administered with Blend 2, and this effect was associated with intestinal microbiota remodeling and increased formation of lactate, butyrate, and propionate. None of these effects were observed in mice administered with Blend 1 (VSL#3). In summary, we have shown that two probiotic mixtures obtained by combining taxonomically similar species produced with different manufacturing methods exert divergent effects in mouse models of colitis.

【 授权许可】

Unknown