The copy-number and varied strengths of MELT motifs in Spc105 balance the strength and responsiveness of the spindle assembly checkpoint

eLife

Simon JY Han¹  Ajit P Joglekar¹  Adrienne Nicole Fontan¹  Babhrubahan Roy¹ 

[1] Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, United States;

关键词: mitosis;    spindle assembly checkpoint;    cell cycle;    chromosome segregation;   

DOI  :  10.7554/eLife.55096

来源: DOAJ

【 摘 要 】

During mitosis, the Spindle Assembly Checkpoint (SAC) maintains genome stability while also ensuring timely anaphase onset. To maintain genome stability, the SAC must be strong to delay anaphase even if just one chromosome is unattached, but for timely anaphase onset, it must promptly respond to silencing mechanisms. How the SAC meets these potentially antagonistic requirements is unclear. Here we show that the balance between SAC strength and responsiveness is determined by the number of ‘MELT’ motifs in the kinetochore protein Spc105/KNL1 and their Bub3-Bub1 binding affinities. Many strong MELT motifs per Spc105/KNL1 minimize chromosome missegregation, but too many delay anaphase onset. We demonstrate this by constructing a Spc105 variant that trades SAC responsiveness for much more accurate chromosome segregation. We propose that the necessity of balancing SAC strength and responsiveness drives the dual evolutionary trend of the amplification of MELT motif number, but degeneration of their functionally optimal amino acid sequence.

【 授权许可】

Unknown