Isolated Murine Brain Model for Large-Scale Optoacoustic Calcium Imaging

Frontiers in Neuroscience

Benedict Mc Larney²  Sven Gottschalk³  Oleksiy Degtyaruk³  Johannes Rebling⁴  Xosé Luis Deán-Ben⁴  Daniel Razansky⁴  Shy Shoham⁵ 

[1] Faculty of Medicine, Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland;Faculty of Medicine, Technical University of Munich, Munich, Germany;Institute for Biological and Medical Imaging, Helmholtz Center Munich, Neuherberg, Germany;Institute for Biomedical Engineering and Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich, Switzerland;Tech4Health and Neuroscience Institutes and Department of Ophthalmology, New York University Langone Health, New York, NY, United States;

关键词: isolated brain;    calcium dynamics;    optoacoustic neuroimaging;    functional neuroimaging;    GCaMP6f;   

DOI  :  10.3389/fnins.2019.00290

来源: DOAJ

【 摘 要 】

Real-time visualization of large-scale neural dynamics in whole mammalian brains is hindered with existing neuroimaging methods having limited capacity when it comes to imaging large tissue volumes at high speeds. Optoacoustic imaging has been shown to be capable of real-time three-dimensional imaging of multiple cerebral hemodynamic parameters in rodents. However, optoacoustic imaging of calcium activity deep within the mammalian brain is hampered by strong blood absorption in the visible light spectrum as well as a lack of activity labels excitable in the near-infrared window. We have developed and validated an isolated whole mouse brain preparation labeled with genetically encoded calcium indicator GCaMP6f, which can closely resemble in vivo conditions. An optoacoustic imaging system coupled to a superfusion system was further designed and used for rapid volumetric monitoring of stimulus-evoked calcium dynamics in the brain. These new imaging setup and isolated preparation’s protocols and characteristics are described here in detail. Our new technique captures calcium fluxes as true three-dimensional information across the entire brain with temporal resolution of 10 ms and spatial resolution of 150 μm, thus enabling large-scale neural recording at penetration depths and spatio-temporal resolution scales not covered with any existing neuroimaging techniques.

【 授权许可】

Unknown