Evidence for multiple-insecticide resistance in urban Aedes albopictus populations in southern China

Parasites & Vectors

Yiji Li¹  Wenqiang Yang¹  Xiao-Guang Chen¹  Yang Wu¹  Kun Wu¹  Jiabao Xu¹  Guiyun Yan¹  Hong Zhang¹  Xinghua Su¹  Songwu Cai²  Guofa Zhou³  Daibin Zhong³ 

[1] Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University;Department of Vector Control, Centers for Disease Control and Prevention of Guangdong Province;Program in Public Health, School of Medicine, University of California;

关键词: Aedes albopictus;    Insecticide resistance;    Biochemical assay;    Kdr;    Urbanization;    Guangzhou;   

DOI  :  10.1186/s13071-017-2581-y

来源: DOAJ

【 摘 要 】

Abstract Background Aedes albopictus (Skuse) is an invasive mosquito that has become an important vector of chikungunya, dengue and Zika viruses. In the absence of specific antiviral therapy or a vaccine, vector management is the sole method available for reducing Aedes-induced disease morbidity. Determining the resistance status of Ae. albopictus to insecticides and exploring the resistance mechanisms is essential for future vector control planning. Methods Aedes albopictus larvae and pupae were sampled from six sites (two sites each from urban, suburban and rural) in Guangzhou. The resistance bioassays were conducted against Bacillus thuringiensis israelensis (Bti): deltamethrin, propoxur and malathion for larvae; and deltamethrin, DDT, propoxur and malathion for adults. P450 monooxygenase (P450s), glutathione S-transferase (GSTs) and carboxylesterase (COEs) activities of adult mosquitoes were measured. Mutations at the knockdown resistance (kdr) gene were analyzed, and the association between kdr mutations and phenotypic resistance was tested. Results Adult bioassays revealed varied susceptibility against DDT, deltamethrin and propoxur in the six Ae. albopictus populations. Significantly lower mortality rates were found in urban populations than suburban and rural populations. Urban mosquito populations showed resistance against DDT, deltamethrin and propoxur, while one rural population was resistant to DDT. All populations tested were susceptible to malathion. Larval bioassays results indicated that all populations of Ae. albopictus were sensitive to the larvicide Bti and malathion. Resistance to deltamethrin and propoxur was common in larval populations. The F1534S and F1534 L mutations were found to be significantly associated with deltamethrin resistance. Biochemical assays indicated elevated detoxification enzyme activities in the field mosquito populations. Conclusions Aedes albopictus populations in Guangzhou, especially in urban areas, have developed resistance to the commonly used insecticides, primarily DDT and deltamethrin. This finding calls for resistance management and developing counter measures to mitigate the spread of resistance.

【 授权许可】

Unknown