The Diversity, Multiplicity of Infection and Population Structure of P. falciparum Parasites Circulating in Asymptomatic Carriers Living in High and Low Malaria Transmission Settings of Ghana

Genes

Zakaria Abukari¹  SamsonP. Salifu¹  CheikhC. Dieng²  Eugenia Lo²  AminataC. Lo³  LindaE. Amoah³  Ruth Okonu³  BenA. Gyan³  SamuelB. Nyarko⁴ 

[1] Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Department of Biological Sciences, University of North Carolina at Charlotte, NC 28223, USA;Immunology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana;School of Medical Sciences, University of Cape Coast, Cape Coast, Ghana;

关键词: MSP2 genotyping;    microsatellites;    parasite;    genetic diversity;    malaria;    multiplicity of infection;   

DOI  :  10.3390/genes10060434

来源: DOAJ

【 摘 要 】

Background: Diversity in Plasmodium falciparum poses a major threat to malaria control and elimination interventions. This study utilized 12 polymorphic microsatellite (MS) markers and the Msp2 marker to examine diversity, multiplicity of infection (MOI) as well as the population structure of parasites circulating in two sites separated by about 92 km and with varying malaria transmission intensities within the Greater Accra Region of Ghana. Methods: The diversity and MOI of P. falciparum parasites in 160 non-symptomatic volunteers living in Obom (high malaria transmission intensity) and Asutsuare (low malaria transmission intensity) aged between 8 and 60 years was determined using Msp2 genotyping and microsatellite analysis. Results: The prevalence of asymptomatic P. falciparum carriers as well as the parasite density of infections was significantly higher in Obom than in Asutsuare. Samples from Asutsuare and Obom were 100% and 65% clonal, respectively, by Msp2 genotyping but decreased to 50% and 5%, respectively, when determined by MS analysis. The genetic composition of parasites from Obom and Asutsuare were highly distinct, with parasites from Obom being more diverse than those from Asutsuare. Conclusion: Plasmodium falciparum parasites circulating in Obom are genetically more diverse and distinct from those circulating in Asutsuare. The MOI in samples from both Obom and Asutsuare increased when assessed by MS analysis relative to MSP2 genotyping. The TA40 and TA87 loci are useful markers for estimating MOI in high and low parasite prevalence settings.

【 授权许可】

Unknown