Production of Bioactive Recombinant Reteplase by Virus-Based Transient Expression System in Nicotiana benthamiana

Frontiers in Plant Science

Ting Ma¹  Sheng Wang²  Zhiying Li² 

[1] Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in the Western China, School of Life Science, Ningxia Universisty, Yinchuan, China;Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia, School of Life Science, Ningxia Universisty, Yinchuan, China;

关键词: Reteplase;    plant viral expression system;    Nicotiana benthamiana;    transient protein production;    Strep-tag II;    fibrinolysis activity;   

DOI  :  10.3389/fpls.2019.01225

来源: DOAJ

【 摘 要 】

To explore a cost-effective alternative method to produce the recombinant thrombolytic drug Reteplase (rPA), a plant viral amplicon-based gene expression system was employed to transiently express bioactive Strep II-tagged recombinant rPA in Nicotiana benthamiana leaves via agro-infiltration. Several gene expression cassettes were designed, synthesized in vitro, and then cloned into Tobacco mosaic virus RNA-based overexpression vector. Codon optimization, subcellular targeting, and the effect of attached Strep-tag II were assessed to identify conditions that maximized expression levels of the recombinant rPA in tobacco leaves. We found that codon-optimized rPA with N-terminal Strep-tag II that was aimed to the endoplasmic reticulum as target provided the highest amount of biologically active protein, i.e., up to ∼50 mg from per kilogram fresh weight leaf biomass in less than 1 week. Furthermore, the recombinant rPA was conveniently purified from inoculated leaf extracts by a one-step purification procedure via the Strep-tag II. The plant-made rPA was glycosylated with molecular mass of ∼45.0 kDa, and its in vitro fibrinolysis activity was equivalent to the commercial available rPA. These results indicate that the plant viral amplicon-based system offers a simple and highly effective approach for cost-effective large-scale production of recombinant rPA.

【 授权许可】

Unknown