期刊论文详细信息
Frontiers in Bioengineering and Biotechnology | |
FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins in Drosophila S2 Cells | |
Michael Fairhead1  Nicola A. Burgess-Brown1  Wyatt W. Yue1  Vittorio L. Katis1  Opher Gileadi1  Willem A. de Jongh2  Stine B. Clemmensen2  Bent U. Frandsen2  Anja Schwenzer3  Brian D. Marsden3  Jesse A. Coker3  Kim S. Midwood3  | |
[1]Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom | |
[2]ExpreS2ion Biotechnologies, SCION-DTU Science Park, Hørsholm, Denmark | |
[3]Kennedy Institute of Rheumatology, University of Oxford, Oxford, United Kingdom | |
关键词: insect cells; recombinant proteins; S2 cells; glycoproteins; secreted proteins; structural biology; | |
DOI : 10.3389/fbioe.2022.871933 | |
来源: DOAJ |
【 摘 要 】
Recombinant protein expression in eukaryotic insect cells is a powerful approach for producing challenging targets. However, due to incompatibility with standard baculoviral platforms and existing low-throughput methodology, the use of the Drosophila melanogaster “S2” cell line lags behind more common insect cell lines such as Sf9 or High-Five™. Due to the advantages of S2 cells, particularly for secreted and secretable proteins, the lack of a simple and parallelizable S2-based platform represents a bottleneck, particularly for biochemical and biophysical laboratories. Therefore, we developed FAS2FURIOUS, a simple and rapid S2 expression pipeline built upon an existing low-throughput commercial platform. FAS2FURIOUS is comparable in effort to simple E. coli systems and allows users to clone and test up to 46 constructs in just 2 weeks. Given the ability of S2 cells to express challenging targets, including receptor ectodomains, secreted glycoproteins, and viral antigens, FAS2FURIOUS represents an attractive orthogonal approach for protein expression in eukaryotic cells.【 授权许可】
Unknown