Graphene Oxide Functional Nanohybrids with Magnetic Nanoparticles for Improved Vectorization of Doxorubicin to Neuroblastoma Cells

Pharmaceutics

Luigi Lerra¹  Florida Voli¹  Orazio Vittorio¹  Marion Le Grand¹  Annafranca Farfalla²  Manuela Curcio²  Giuseppe Cirillo²  FiorePasquale Nicoletta²  Francesca Iemma²  GerardoF. Goya³  Silke Hampel⁴  Anna Dubrovska⁵  Beatriz Sanz⁶ 

[1] Children’s Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, NSW 2031, Australia;Department of Pharmacy Health and Nutritional Science, University of Calabria, 87036 Rende (CS), Italy;Institute of Nanoscience of Aragon (INA), Department of Condensed Matter Physics, University of Zaragoza, 50018 Zaragoza, Spain;Leibniz Institute of Solid State and Material Research Dresden, 01069 Dresden, Germany;OncoRay—National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany;nB nanoSacale Biomagnetics SL, 50012 Zaragoza, Spain;

关键词: graphene oxide;    iron oxide nanoparticles;    magnetic targeting;    nanohybrids;    synergism;   

DOI  :  10.3390/pharmaceutics11010003

来源: DOAJ

【 摘 要 】

With the aim to obtain a site-specific doxorubicin (DOX) delivery in neuroblastoma SH-SY5Y cells, we designed an hybrid nanocarrier combining graphene oxide (GO) and magnetic iron oxide nanoparticles (MNPs), acting as core elements, and a curcumin–human serum albumin conjugate as functional coating. The nanohybrid, synthesized by redox reaction between the MNPs@GO system and albumin bioconjugate, consisted of MNPs@GO nanosheets homogeneously coated by the bioconjugate as verified by SEM investigations. Drug release experiments showed a pH-responsive behavior with higher release amounts in acidic (45% at pH 5.0) vs. neutral (28% at pH 7.4) environments. Cell internalization studies proved the presence of nanohybrid inside SH-SY5Y cytoplasm. The improved efficacy obtained in viability assays is given by the synergy of functional coating and MNPs constituting the nanohybrids: while curcumin moieties were able to keep low DOX cytotoxicity levels (at concentrations of 0.44–0.88 µM), the presence of MNPs allowed remote actuation on the nanohybrid by a magnetic field, increasing the dose delivered at the target site.

【 授权许可】

Unknown