Functionalization of Bulk SiO2 Surface with Biomolecules for Sensing Applications: Structural and Functional Characterizations

Chemosensors

Sebania Libertino¹  Corrado Bongiorno¹  EmanueleLuigi Sciuto¹  Tiziana Cosentino²  Fulvia Sinatra²  Antonino Scandurra³  Salvatore Petralia⁴  Sabrina Conoci⁴ 

[1] CNR-IMM Headquarters, Strada VIII Z.I. 5, 95121 Catania, Italy;Department of Biomedical and Biotechnological Sciences, Via S. Sofia 87, 95100 Catania, Italy;Department of Physics and Astronomy, University of Catania, 95100 Catania, Italy;STMicroelectronics, Stradale Primosole 50, 95121 Catania, Italy;

关键词: biointerface;    silicon;    ssDNA;    glucose oxidase;    horseradish peroxide;    metallothionein;    TEM;    XPS;   

DOI  :  10.3390/chemosensors6040059

来源: DOAJ

【 摘 要 】

Biomolecule immobilization on bulk silicon dioxide (SiO₂) is an important aspect in the field of Si-based interfaces for biosensing. The approach used for surface preparation should guarantee not only the stable anchoring of biomolecules but also their structural integrity and biological functioning. In this paper, we review our findings on the SiO₂ functionalization process to immobilize a variety of biomolecules, including glucose oxidase, horseradish peroxide, metallothionein, and DNA molecules. Morphological and chemical characterization of SiO₂ surfaces after biomolecule immobilization using techniques already employed in the microelectronic industry are presented and discussed. Optical and spectrophotometric analysis revealed the preservation of biomolecules’ activity once they are anchored on the biointerface.

【 授权许可】

Unknown