Cellulose Nanofiber-Reinforced Chitosan Hydrogel Composites for Intervertebral Disc Tissue Repair

Biomimetics

Carlos Peniche-Covas¹  Christian Gorzelanny²  Laurent David³  Alexandra Montembault³  Guillaume Sudre³  Tuan Ahn Tran⁴  Ingo Doench⁴  Malika Louback-Mohamed⁴  Anayancy Osorio-Madrazo⁴  Thibaut Cachon⁵  Eric Viguier⁵  Niels Horbelt⁶ 

[1] Center of Biomaterials, University of Havana, 10600 Havana, Cuba;Department of Dermatology and Venerology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany;Ingénierie des Matériaux Polymères (IMP), CNRS UMR 5223, Université Claude Bernard Lyon 1, Université de Lyon, 69622 Villeurbanne CEDEX, France;Institute of Microsystems Engineering IMTEK, Laboratory for Sensors, University of Freiburg, 79110 Freiburg, Germany;Interaction Cells Environment (ICE), VetAgro Sup, Université de Lyon, 69280 Marcy l’Etoile, France;Max-Planck Institute of Colloids and Interfaces, Biomaterials Department, Science Park Golm, 14476 Potsdam, Germany;

关键词: hydrogel biomaterial;    chitosan;    cellulose nanofibers;    intervertebral disc;    tissue engineering;   

DOI  :  10.3390/biomimetics4010019

来源: DOAJ

【 摘 要 】

The development of non-cellularized composites of chitosan (CHI) hydrogels, filled with cellulose nanofibers (CNFs) of the type nanofibrillated cellulose, was proposed for the repair and regeneration of the intervertebral disc (IVD) annulus fibrosus (AF) tissue. With the achievement of CNF-filled CHI hydrogels, biomaterial-based implants were designed to restore damaged/degenerated discs. The structural, mechanical and biological properties of the developed hydrogel composites were investigated. The neutralization of weakly acidic aqueous CNF/CHI viscous suspensions in NaOH yielded composites of physical hydrogels in which the cellulose nanofibers reinforced the CHI matrix, as investigated by means of microtensile testing under controlled humidity. We assessed the suitability of the achieved biomaterials for intervertebral disc tissue engineering in ex vivo experiments using spine pig models. Cellulose nanofiber-filled chitosan hydrogels can be used as implants in AF tissue defects to restore IVD biomechanics and constitute contention patches against disc nucleus protrusion while serving as support for IVD regeneration.

【 授权许可】

Unknown