Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Enterobacter spp. isolated from different clinical samples in Tehran, Iran

Revista da Sociedade Brasileira de Medicina Tropical

关键词: Enterobacter;    ESBL;    AmpC;    RAPD-PCR;   

DOI  :  10.1590/0037-8682-0227-2017

来源: DOAJ

【 摘 要 】

AbstractINTRODUCTION:Here, we determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples.METHODS:A total of 57 clinical isolates of Enterobacter spp. were tested for the production of extended-spectrum β-lactamases (ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods.RESULTS:The most common ESBLs and AmpC β-lactamases were bla TEM (63.3%) and bla EBC (57.7%), respectively. The most prevalent virulence gene was rpos (87.7%). The random amplified polymorphic DNA (RAPD) patterns of strains were genetically unrelated.CONCLUSIONS:RAPD polymerase chain reaction analysis revealed high genetic diversity among isolates.

【 授权许可】

Unknown