First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in Argentina

BMC Veterinary Research

Ramon Sanguinetti¹  Javier Alejandro Cappuccio²  Laura Valeria Alarcón³  Maria Alejandra Quiroga⁴  Alberto Armocida⁴  Maria Inez Lozada⁴  Estefanía Marisol Pérez⁴  Carlos Juan Perfumo⁴  Pablo Enrique Piñeyro⁵  Darin Michael Madson⁵  Fabio Vannucci⁶ 

[1] DILACOT-SENASA;EEA Marcos Juaréz, INTA, CONICET;HIPRA Argentina;Laboratorio de Patología Especial Veterinaria FCV-UNLP;Veterinary Diagnostic Laboratory, 1655 Veterinary Medicine, Iowa State University;Veterinary Diagnostic Laboratory, University of Minnesota;

关键词: Porcine transmissible gastroenteritis virus;    Diarrhea;    Mortality;    Piglets;   

DOI  :  10.1186/s12917-018-1615-9

来源: DOAJ

【 摘 要 】

Abstract Background In 2014, a notification of porcine transmissible gastroenteritis virus (TGEV) was made by the National Services of Animal Health of Argentina (SENASA) to the World Organization of Animal Health (OIE). The notification was based on a serological diagnosis in a small farm with a morbidity rate of 2.3% without enteric clinical signs. In order to determine if TGEV was circulating before the official report, a retrospective study on cases of neonatal diarrhea was performed. The selection criteria was a sudden increase in mortality in 1- to 21-day-old piglets with watery diarrhea that did not respond to antibiotics. Based on these criteria, three clinical cases were identified during 2010–2015. Results All animals that were evaluated presented histological lesions consistent with enteric viral infection. The feces and ultrathin sections of intestine that were evaluated by electron microscopy confirmed the presence of round particles of approximately 80 nm in size and characterized by finely granular electrodense nucleoids consistent with complete particles of coronavirus. The presence of the TGEV antigen was confirmed by monoclonal specific immunohistochemistry, and final confirmation of a metabolically-active virus was performed by in situ hybridization to detect a TGE mRNA encoding spike protein. All sections evaluated in this case were negative for PEDV and rotavirus A. Conclusions This is the first case series describing neonatal mortality with etiological confirmation of TGEV in Argentina. The clinical diagnosis of TGEV infections in endemic regions is challenging due to the epidemiological distribution and coinfection with other enteric pathogens that mask the clinical presentation.

【 授权许可】

Unknown