Olive Mill and Olive Pomace Evaporation Pond’s By-Products: Toxic Level Determination and Role of Indigenous Microbiota in Toxicity Alleviation

Applied Sciences

Lenka Luptakova¹  Tomasz Oszako²  Lassaad Belbahri³  Armelle Vallat⁴  HoudaBen Slama⁵  Ameur Khardani⁵  AliChenari Bouket⁶  MostafaE. Rateb⁷  FaizahN. Alenezi⁸ 

[1] Department of Biology and Genetics, Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, 04181 Kosice, Slovakia;Department of Forest Protection of the Forest Research Institute in Sekocin Stary, 05-090 Raszyn, Poland;Laboratory of Soil Biology, University of Neuchatel, 2000 Neuchatel, Switzerland;Neuchatel Platform of Analytical Chemistry, Institute of Chemistry, University of Neuchâtel, 2000 Neuchâtel, Switzerland;NextBiotech, 98 Rue Ali Belhouane, Agareb 3030, Tunisia;Plant Protection Research Department, East Azarbaijan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Tabriz 5355179854, Iran;School of Computing, Engineering & Physical Sciences, University of the West of Scotland, Paisley PA1 2BE, UK;The Public Authority for Applied Education and Training, Adailiyah 00965, Kuwait;

关键词: OMW;    OMP;    evaporation pond;    heavy metals;    LC-HRMS;    metagenomics;   

DOI  :  10.3390/app11115131

来源: DOAJ

【 摘 要 】

Diverse vegetable oils are extracted from oleagenic trees and plants all over the world. In particular, olive oil represents a strategic socio-economic branch in the Mediterranean countries. These countries use either two or three-phase olive oil extraction systems. In this work, we focus on the by-products from three-phase olive oil extraction, which are the liquid olive mill wastewater (OMW) and the solid olive mill pomace (OMP) rejected in evaporative ponds. The disposal of this recalcitrant waste poses environmental problems such as the death of different species of insects and animals. In-depth ICP-OES analysis of the heavy metal composition of OMW and OMP revealed the presence of many metals ranging from non-toxic to highly toxic. The LC-HRMS characterization of these by-products indicated the presence of several secondary metabolites harmful to humans or to the environment. Thus, we aimed to identify OMW and OMP indigenous microbiota through metagenomics. The bacterial population was dominated by the Acetobacter (49.7%), Gluconobacter (17.3%), Gortzia (13.7%) and Nardonalla (5.3%) genera. The most abundant fungal genera were Nakazawaea, Saccharomyces, Lachancea and Candida. These microbial genera are responsible for OMW, OMP and soil toxicity alleviation.

【 授权许可】

Unknown