Exo-ethylene application mitigates waterlogging stress in soybean (Glycine max L.)

BMC Plant Biology

Jeung-Woo Ko¹  Chang-Woo Seo¹  Bong-Gyu Mun¹  Byung-Wook Yun¹  Soon-Ki Park¹  Raheem Shahzad¹  In-Jung Lee¹  Yoonha Kim¹  Abdul Latif Khan² 

[1] Division of Plant Biosciences, Kyungpook National University;UoN Chair of Oman’s Medicinal Plants & Marine Natural Products, University of Nizwa;

关键词: Antioxidant;    Gibberellin;    Photosynthesis efficiency;    Plant growth regulator;    Reactive nitrogen species;    Reactive oxygen species;   

DOI  :  10.1186/s12870-018-1457-4

来源: DOAJ

【 摘 要 】

Abstract Background Waterlogging (WL) is a key factor hindering soybean crop productivity worldwide. Plants utilize various hormones to avoid various stress conditions, including WL stress; however, the physiological mechanisms are still not fully understood. Results To identify physiological mechanisms during WL stress, different phytohormones, such as ethephon (ETP; donor source of ethylene), abscisic acid, gibberellins, indole-3-acetic acid, kinetin, jasmonic acid, and salicylic acid were exogenously applied to soybean plants. Through this experiment, we confirmed the beneficial effects of ETP treatment. Thus, we selected ETP as a candidate hormone to mitigate WL. Further mechanistic investigation of the role of ETP in waterlogging tolerance was carried out. Results showed that ETP application mitigated WL stress, significantly improved the photosynthesis pigment, and increased the contents of endogenous GAs compared to those in untreated plants. The amino acid contents during WL stress were significantly activated by EPT treatments. The amino acid contents were significantly higher in the 100 μM ETP-treated soybean plants than in the control. ETP application induced adventitious root initiation, increased root surface area, and significantly increased the expressions of glutathione transferases and relative glutathione activity compared to those of non-ETP-treated plants. ETP-treated soybeans produced a higher up-regulation of protein content and glutathione S-transferase (GSTs) than did soybeans under the WL only treatment. Conclusions In conclusion, the current results suggest that ETP application enabled various biochemical and transcriptional modulations. In particular, ETP application could stimulate the higher expression of GST3 and GST8. Thus, increased GST3 and GST8 induced 1) increased GSH activity, 2) decreased reactive oxygen species (ROS), 3) mitigation of cell damage in photosynthetic apparatus, and 4) improved phenotype consecutively.

【 授权许可】

Unknown