Label-Free Optical Biosensing Using Low-Cost Electrospun Polymeric Nanofibers

Chemosensors

Inés Peraile¹  Paula Martínez-Pérez²  Salvador Ponce-Alcántara²  Jaime García-Rupérez²  Nieves Murillo³  Jon Maudes³  Ana Pérez-Márquez³  Matilde Gil-García⁴  Paloma Lorenzo-Lozano⁴  Laura González-López⁴ 

[1] Ingeniería de Sistemas para la Defensa de España (ISDEFE), 28040 Madrid, Spain;Nanophotonics Technology Center, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain;TECNALIA Research & Innovation, Mikeletegi Pasealekua, 2, 20009 Donostia-San Sebastián, Spain;Área de Defensa Biológica, Departamento de Sistemas de Defensa NBQ, Instituto Nacional de Técnica Aeroespacial “Esteban Terradas” (INTA)-Campus La Marañosa, Crta. San Martín de la Vega (M−301), km 10.5, 28330 Madrid, Spain;

关键词: nanofibers;    porous materials;    Fabry–Pérot cavity;    optical biosensor;    label-free biosensing;   

DOI  :  10.3390/chemosensors8040119

来源: DOAJ

【 摘 要 】

Polymeric nanofiber matrices are promising structures to develop biosensing devices due to their easy and affordable large-scale fabrication and their high surface-to-volume ratio. In this work, the suitability of a polyamide 6 nanofiber matrix for the development of a label-free and real-time Fabry–Pérot cavity-based optical biosensor was studied. For such aim, in-flow biofunctionalization of nanofibers with antibodies, bound through a protein A/G layer, and specific biodetection of 10 µg/mL bovine serum albumin (BSA) were carried out. Both processes were successfully monitored via reflectivity measurements in real-time without labels and their reproducibility was demonstrated when different polymeric nanofiber matrices from the same electrospinning batch were employed as transducers. These results demonstrate not only the suitability of correctly biofunctionalized polyamide 6 nanofiber matrices to be employed for real-time and label-free specific biodetection purposes, but also the potential of electrospinning technique to create affordable and easy-to-fabricate at large scale optical transducers with a reproducible performance.

【 授权许可】

Unknown