Comparative study on the viability of Vero cells stored under various levels of cryopreservation variables

Dysona. Life Science

MN Islam¹  SMZH Chowdhury²  M Giasuddin³  MSI Siddiqui⁴  EH Chowdhury⁵  Jahan Ara Begum⁵  MR Islam⁵ 

[1] Animal Production Division, Department of Livestock Services, Government of People’s Republic of Bangladesh, Dhaka, Bangladesh;Bangladesh Agricultural Research Council, Farmgate, Dhaka, Bangladesh;Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh;Department of Anatomy and Histology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Bangladesh;Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Bangladesh;

关键词: cryoprotectant;    slow freezing;    deep freezing;   

DOI  :  10.30493/dls.2022.327063

来源: DOAJ

【 摘 要 】

The effects of different preservation temperatures two cryoprotectants (glycerol and DMSO) at two concentrations (5% and 10%), followed by two different freezing methods (rapid and slow freezing) and two different preservation temperatures (-80°C and -196°C) on the viability of Vero cells were examined in this study. Vero cells were preserved for 18 months under the combinations of the aforementioned treatments. After thawing, the revivability and viability of the stored samples were evaluated. The highest cells revivability and cells viability were found in 10% glycerol treatment combined with the slow freezing method and followed by cryopreservation. In contrast, the lowest revivability and viability values were observed in the combinations of DMSO as a cryoprotectant and rapid freezing method, regardless of DMSO concentration and preservation method. It can be concluded that using 10% glycerol as cryoprotectant followed by the reported slow freezing protocol and deep freezing or, more preferably, cryopreservation may be a suitable method in cryobiology for the long term Vero cells preservation. However, more research should be carried out to optimize cryoprotectant concentration in the preservation medium.

【 授权许可】

Unknown